get rid of Alkaline phosphatase

Sue Jin sjin at STANFORD.EDU
Wed Sep 8 17:08:21 EST 1999


Thank you for all the helps. The phosphatase I am using is SAP. However, I
phoned the Roche company where we bought the enzyme from. They suggested me
to do the phenol extraction for the ligation since ligation is picky
sometimes. Does anybody have experience with SAP without doing phenol
extraction and also have good result of ligation? thanks!!

sue



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