get rid of Alkaline phosphatase

Junzhi Wei jzwei at CC.USU.EDU
Wed Sep 8 19:10:27 EST 1999


Hi. Sue:

Try QIAquick spin column. It works well for getting rid of alkaline
phosphatase.

J. Wei

At 03:08 PM 9/8/99 -0700, Sue Jin wrote:
>Thank you for all the helps. The phosphatase I am using is SAP. However, I
>phoned the Roche company where we bought the enzyme from. They suggested me
>to do the phenol extraction for the ligation since ligation is picky
>sometimes. Does anybody have experience with SAP without doing phenol
>extraction and also have good result of ligation? thanks!!
>
>sue
>
>
Jun-zhi Wei
Forage and Range Research Lab
Utah State University
Logan, UT 84322-6300
jzwei at cc.usu.edu



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