get rid of Alkaline phosphatase

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Thu Sep 9 12:27:16 EST 1999


Junzhi Wei wrote:
> 
> Hi. Sue:
> 
> Try QIAquick spin column. It works well for getting rid of alkaline
> phosphatase.
> 
> J. Wei
> 
> At 03:08 PM 9/8/99 -0700, Sue Jin wrote:
> >Thank you for all the helps. The phosphatase I am using is SAP. However, I
> >phoned the Roche company where we bought the enzyme from. They suggested me
> >to do the phenol extraction for the ligation since ligation is picky
> >sometimes. Does anybody have experience with SAP without doing phenol
> >extraction and also have good result of ligation? thanks!!
> >
> >sue
> >
> >
> Jun-zhi Wei
> Forage and Range Research Lab
> Utah State University
> Logan, UT 84322-6300
> jzwei at cc.usu.edu

I also use the Qiaquick column, works pretty well. 

Ricky



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