how to amplify a GC-rich DNA by PCR

[Yu Wai Chen]

Don Walthers wrote:
> 
> i find that extended denaturing and annealing times (2 minutes) help. for my
> primers 95C and 48C work for denaturning and annealing temps. also try %5
> DMSO.
> 

You may also try adding betaine.

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Yu Wai CHEN, Ph.D. ..................   email:ywc at mrc-lmb.cam.ac.uk
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