how to amplify a GC-rich DNA by PCR

Yu Wai Chen ywc at mrc-lmb.cam.ac.uk
Fri Sep 10 07:18:41 EST 1999


Don Walthers wrote:
> 
> i find that extended denaturing and annealing times (2 minutes) help. for my
> primers 95C and 48C work for denaturning and annealing temps. also try %5
> DMSO.
> 

You may also try adding betaine.

-- 
===================================================================
Yu Wai CHEN, Ph.D. ..................   email:ywc at mrc-lmb.cam.ac.uk
 Centre for Protein Engineering,             tel:+44-(0)1223-402148
 MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140
 WWW homepage: http://www.mrc-cpe.cam.ac.uk/~ywc



More information about the Methods mailing list