how to amplify a GC-rich DNA by PCR

Yu Wai Chen ywc at
Fri Sep 10 07:18:41 EST 1999

Don Walthers wrote:
> i find that extended denaturing and annealing times (2 minutes) help. for my
> primers 95C and 48C work for denaturning and annealing temps. also try %5

You may also try adding betaine.

Yu Wai CHEN, Ph.D. ..................   email:ywc at
 Centre for Protein Engineering,             tel:+44-(0)1223-402148
 MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140
 WWW homepage:

More information about the Methods mailing list