get rid of Alkaline phosphatase

[Richard P. Grant]

In article <4.1.19990908085927.009159f0 at sjin.pobox.stanford.edu>,
sjin at STANFORD.EDU (Sue Jin) wrote:


>I am wondering  if  there is a easy way to get rid of the posphatase
>without doing phenol extraction after dephosphorylation of the plasmid DNA
>for subcloning? Your help is appreciated!!

Sure, use Shrimp Alk phos from USB - heat sensitive, no organic required.

HTH,

Richard

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
work: www.cmtech.co.uk        |      home: www.avnet.co.uk/adastra           
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