get rid of Alkaline phosphatase
Richard P. Grant
r_grant at see.sig.for.address
Fri Sep 10 10:27:39 EST 1999
In article <4.1.19990908085927.009159f0 at sjin.pobox.stanford.edu>,
sjin at STANFORD.EDU (Sue Jin) wrote:
>I am wondering if there is a easy way to get rid of the posphatase
>without doing phenol extraction after dephosphorylation of the plasmid DNA
>for subcloning? Your help is appreciated!!
Sure, use Shrimp Alk phos from USB - heat sensitive, no organic required.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
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