Immunoprecipitations??

Neal Melvin nrmelvin at acs.ucalgary.ca
Sun Sep 12 18:48:19 EST 1999


I'm doing IPs with 20 ul of a 50% protein A-Sepahrose in a total volume
of 1 ml of lysis buffer... I find that I can barely see a 'pellet'
forming after centrifugation. Am I using too little protein
A-Sepharose??
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