test for bacterial inclusion bodies?

soenke behrends behrends at plexus.uke.uni-hamburg.de
Mon Sep 13 04:40:49 EST 1999


Dear netters,

when you express a protein in E. coli 
and you get the impression that you 
have inclusion bodies. Is there a 
quick method for diagnosis? How do 
you test whether the protein just sticks
to the membrane or whether you have 
inclusion bodies?

Thanks for your time and help
Soenke








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