chrisb at hgu.mrc.ac.uk
Mon Sep 13 05:06:57 EST 1999
Chris Small (chris at GENOMEX.COM) wrote:
: I'm hoping someone can tell me why my home-made electrocompetent
: DH10B cells produce both dark blue colonies and very light (almost
: white) colonies when transformed with a BAC vector (pBELOBAC 11)
: while the commercial cells yield uniformly blue transformants with
: the same vector. I want to make my own cells but they don't look
One possibility is that your cells (or cuvettes) are contaminated with
DNA of a (high copy number) plasmid vector, a far commoner problem than
it ought to be. To test this, try electroporating without adding DNA.
If you get any dark blues, you need to re-make the competent cells.
Chris Boyd | from (but not \ MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk | much longer) / Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd EH4 2XU, SCOTLAND
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