test for bacterial inclusion bodies?

Jayakumar R jakku at mrna.tn.nic.in
Mon Sep 13 08:07:55 EST 1999

  It is easy.  Use phase contrast microscope to see the inclusion bodies.
Allow the strain to produce the inclusion bodies and just observe them
under the phase contrast microscope.  That's how we do for Bacillus
thuringiensis cry inclusionbodies.

On Mon, 13 Sep 1999, soenke behrends wrote:

> Dear netters,
> when you express a protein in E. coli 
> and you get the impression that you 
> have inclusion bodies. Is there a 
> quick method for diagnosis? How do 
> you test whether the protein just sticks
> to the membrane or whether you have 
> inclusion bodies?
> Thanks for your time and help
> Soenke


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