Electrocompetent DH10B

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Sep 13 10:02:31 EST 1999


In article <v0421010ab3ff35e97d88@[134.121.168.66]>, Chris Small
<chris at GENOMEX.COM> writes
>I'm hoping someone can tell me why my home-made electrocompetent 
>DH10B cells produce both dark blue colonies and very light (almost 
>white) colonies when transformed with a BAC vector (pBELOBAC 11) 
>while the commercial cells yield uniformly blue transformants with 
>the same vector.  I want to make my own cells but they don't look 
>right.

Contaminated cell stock? Does the morphology of the blue and pale blues
look the same. If you plate the electrocompetent cells on their own do
they all look alike and identical to bona fide DH10B?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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