Why DTT in RNA prep?

Rudi van de Wetering nospamr.vandewetering at czzorlnm.azn.nl
Thu Sep 16 11:40:04 EST 1999


>
>Maybe it is to reduce the proteins such as RNase.
>A final concentration of 1 mM DTT at pH of 8 should be quite effective at
>reducing all proteins.
>
I read that adding DTT (0,5 mM) to cell-lysates prepared for measurement of
B-gal stabilizes its activity. I use it routinely now and it works fine. Why
doesn't B-gal inactivate, or Luciferase for that matter since I measure
both? Don't they have disulfide bonds?





More information about the Methods mailing list