antigen retrieval mouse skin sections

mvooys mvooys at
Fri Sep 17 04:58:34 EST 1999

Dear Colleagues,

When performing IHC on mouse skin tissue sections (formalin fixed),
retrieval (15 min 10 mM citrate buffer Ph6) the dermis disattaches from
glass slides ( silane coated). The epidermis is fine and we get a
signal but clearly we need to improve conditions. Has anyone had similar

problems and suggestions for improving this.


marc vooijs

The Netherlands Cancer Institute
Division of molecular Genetics

mvooys at

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