antigen retrieval mouse skin sections

mvooys mvooys at nki.nl
Fri Sep 17 04:58:34 EST 1999


Dear Colleagues,

When performing IHC on mouse skin tissue sections (formalin fixed),
antigen
retrieval (15 min 10 mM citrate buffer Ph6) the dermis disattaches from
the
glass slides ( silane coated). The epidermis is fine and we get a
specific
signal but clearly we need to improve conditions. Has anyone had similar

problems and suggestions for improving this.

regards,

marc vooijs

The Netherlands Cancer Institute
Division of molecular Genetics

mvooys at nki.nl





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