chenlinan at 263.NET
Mon Sep 20 07:55:39 EST 1999
What buffer I must use when annealing? And what percent of adaptor the
method will acheive?
>> Can two
>> synthesized oligos can be annealed two give adaptors?
>That´s the way, yes. Let synthesize two complementary oligos, mix equal
>amounts, boil for 5 min, then let slowly cool to room temperature. Making
>adaptors from custom oligos is usually cheaper, and much more flexible than
>buying those from companies. You can, e.g. engineer an additional restriction
>site into the adaptors that may be useful later.
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