protein passes through membrane!?

[Ian A. York]

In article <37E5E007.E3666156 at uni-muenster.de>,
Andreas Vogel  <vogela at uni-muenster.de> wrote:
>
>The problem is: the proteins doesnt bind to the membrane but passes
>through it, as I can see for the prestained standard, which I can found

Different lots of product can vary widely.  Try a different lot; you may
have  got a bad batch.

You should also try PVDF rather than nitrocellulose, as a further
variable.

Ian 
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England