yeast three hybrid

Philipp Pagel philipp.pagel at
Tue Sep 21 11:47:22 EST 1999


> Clonetech called SD. In our hands the L40uraMS2 yeast strain which should
> not grow on YC - His medium does grow on SD - His. Is anyone in the UK using

Just an idea you probably had yourself: Is it possible, that your yeast
strain is leaky for HIS3 expression? Maybe you have to use 3-AT
(3-amino-1,2,4-triazole) to compensate.  


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