E.coli growth in minimal medium

Evangelos Christodoulou echristo at biology.db.uoa.gr
Wed Sep 22 02:07:16 EST 1999


Hi there,
We have cloned a gene into the expression vector/host system 
pET15b/BL21(DE3).
We grow the cells in LB and get high amounts of overexpressed
protein in inclusion bodies.
We want to replace LB with a minimal medium like M9.
I understand the medium should be supplemented with amino acids
and vitamins, etc.
Could someone send me a protocol and/or a reference for this?
Any comments are welcome.
TIA,

Van

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Evangelos E. Christodoulou
University of Athens
Biology Department
Division of Biochemistry & Molecular Biology
Biochemistry laboratory
Panepistimiopolis, Zographou
157 01 Athens, GREECE
Tel (office): +30-1-7274510 (direct)
Tel (home) : +30-1-7292770
Fax(office): +30-1-7257572
http://powermac.db.uoa.gr/~Vangelis/index.html
ICQ  29601806
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