PCR

ckf craig at fulton52.freeserve.co.uk
Wed Sep 22 12:11:02 EST 1999


I want to try and PCR up a gene (or part of) using degenerate primers. I
normally use about 20 pmoles of each primer but as the degeneracy of one
primer is about 2000 and the other about 100 do I need to add more than
normal? Any general hints about this sort of PCR would be we useful.

Thanks

Craig





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