chimeric race products???

Gregor st2153 at
Thu Sep 23 05:16:01 EST 1999

Hi everybody!

I´ve tried to race several genes with the Marathon kit. I do nested PCRs
with little or no product in the first round (30 cycles) and many bands
and a smear in the second round 30 cycles as well (with recommended
temperatures 72 X5/70 X5/68 X20). the bands I´ve sequenced so far often
start with the expected bases but then the sequence "hops" to another -
totaly unrelated - sequence.

Do I have to lower (raise) temperatures?

Or lower/raise template concentration?

What are - generally - the parameters that favor for chimeric PCR
products/jumping PCR?

THanks for advice!!

Gregor Bucher, LMU München
st2153 at

the problem with working on the cutting edge is 
that you get sliced from time to time

More information about the Methods mailing list