Ethidium Bromide -Vapors!!!
css at med.unc.edu
Fri Sep 24 10:59:06 EST 1999
let me preface this by saying that i almost always add EtBr after
microwaving the agarose. but i have worked with people who like to make
up 500 ml flasks of agarose with EtBr, which they then nuke every time
they need a gel. The compromise solution was that they, instead of
microwaving the solution with the EtBr in it, instead heated it on low
on a heat plate in the fume hood. Works fine, as long as you don't
crank it on high.
Antonieta Herrera wrote:
> I always melt agorose/TA+Ethidium in a microwave oven. Uhmm a
> lot of steam!! I would like to know how toxic is the emanated
> steam. The oven is outside of the lab air extractor.Is that correct?
> I am still ok, thanks
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