Ethidium Bromide -Vapors!!!
leman at leman.org
Fri Sep 24 13:43:02 EST 1999
On Fri, 24 Sep 1999 11:59:06 -0400, Caroline Szymeczek-Seay
<css at med.unc.edu> wrote:
>let me preface this by saying that i almost always add EtBr after
>microwaving the agarose. but i have worked with people who like to make
>up 500 ml flasks of agarose with EtBr, which they then nuke every time
>they need a gel. The compromise solution was that they, instead of
>microwaving the solution with the EtBr in it, instead heated it on low
>on a heat plate in the fume hood. Works fine, as long as you don't
>crank it on high.
Even better yet: make up a big flask of it and keep it in a 65C
incubator. This way you just don't have to reheat it.
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