try double to 10 fold higher conce ckf wrote: > I want to try and PCR up a gene (or part of) using degenerate primers. I > normally use about 20 pmoles of each primer but as the degeneracy of one > primer is about 2000 and the other about 100 do I need to add more than > normal? Any general hints about this sort of PCR would be we useful. > > Thanks > > Craig