PCR

Chris LaRosa plarosa1 at navix.net
Sat Sep 25 08:39:39 EST 1999


try double to 10 fold higher conce


ckf wrote:

> I want to try and PCR up a gene (or part of) using degenerate primers. I
> normally use about 20 pmoles of each primer but as the degeneracy of one
> primer is about 2000 and the other about 100 do I need to add more than
> normal? Any general hints about this sort of PCR would be we useful.
>
> Thanks
>
> Craig




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