small fragment library construction

[Tom Knight]

chrisb at hgu.mrc.ac.uk (Chris Boyd) writes:

> Homologous recombination in the host will generate the desired
> constructs. Because your inserts are so small, the process could be
> quite efficient. With a suitable design of primers, you could even try
> IVC and conventional cloning with the same PCR fragment pool.

Kids, remember to use a RecA+ strain if you do this at home.