small fragment library construction

Tom Knight tk at pasteur.ai.mit.edu
Tue Sep 28 08:14:39 EST 1999


chrisb at hgu.mrc.ac.uk (Chris Boyd) writes:

> Homologous recombination in the host will generate the desired
> constructs. Because your inserts are so small, the process could be
> quite efficient. With a suitable design of primers, you could even try
> IVC and conventional cloning with the same PCR fragment pool.

Kids, remember to use a RecA+ strain if you do this at home.









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