Cloned, but not expressed.

Hung-yiu Ho hyho at GATE.SINICA.EDU.TW
Tue Sep 28 11:23:34 EST 1999


according to our experience , the expression of GFP in mammalian cells 
depends much on the version of GFP you are using. Please use EGFP or 
Green Lantern from BRL.

On Fri, 20 Aug 1999, Andrew Leung wrote:

> Hi,
> 
>     I have cloned my favorite gene into a fusion gfp vector. But it didn't
> express.
> 
>     My question is that:
> 
> Is translation always start at the first AUG codon the complex first
> encounters? If yes, I don't know why my gfp didn't come up.
> 
> Is there any sequence in the transcript (which upstream to any AUG) that can
> determine whether an AUG could be the starting site for the translation?
> 
> Andrew
> 
> 
> 
> 



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