Ligation inhibiting electroporation?

Scott Henderson scotth at physci.ucla.edu
Tue Sep 28 13:54:08 EST 1999


Are your time constants affected by your ligase rxn?

After ligation I usually add some carrier glycogen, do an organic
extraction, EtOH ppt, and wash 2x w/ 70% EtOH.  Time constants are
typically 4.7 to 4.9.

Scott



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