Ethidium Bromide -Vapors!!!
Mark_Dowton at uow.edu.au
Tue Sep 28 19:02:20 EST 1999
In article <199909280101.LAA07679 at bohm.anu.edu.au>,
s3148487 at STUDENT.ANU.EDU.AU (Brendan Scott) wrote:
I absolutely agree with Bill and Peter's response to this, and I think
both were being too nice. This is completely tasteless, and could only
come from someone who has never had children. Are you observing your
colleague (I wouldn't use 'friend') as a mutagenicity trial? You would
never work in my lab.
> I don't know whether its the EtBr or something else, but one of our
> overzealous technical officers tried making mass amounts of agarose for
> gels for a while, but found that after several rounds of
> remelting/reboiling in the microwave, the agarose would go brown and be
> Incidentally, the technical officer in question is now heavily pregnant,
> and I will be happy to provide a list of birth defects to methods-reagents
> after the delivery.
> >>Second, you destroy part of EtBr by boiling.
> > & many others recycling gels by boiling...
> > I have been told that EtBr is _VERY_ heat stable (above 1000oC)...
> Brendan Scott
> Human Genetics Group
> John Curtin School of Medical Research
> Australian National University
> Canberra ACT 0200
> Ph: (026) 249 0417/3725
> Fax (026) 249 4712
> 0418 871 514
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