Ligation inhibiting electroporation?

John F Mackay MackayJF at bmnz.co.nz
Tue Sep 28 21:48:13 EST 1999


There was a nifty trick (where else but BioTechniques) in  '95 I think. It
was a butanol extraction and used to allow the whole of a ligation to be
electroporated. I used the method but included a 70% ethanol step to remove
the traces of the butanol left. Worked very well



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