Plasmid Toolkit users - help.

Student mucineer at
Wed Sep 29 04:15:49 EST 1999

I was trying to build a multiple cloning site using the "MCS Library"
function in Plasmid Toolkit 2.1. However, one of the enzymes in the MCS is
not in the list of enzymes in the program. Whilst I can add this enzyme
(SbfI) individually by typing in SbfI instead of scrolling down the list
with the "Add restriction site" function, I can't do this as part of the
MCS. Does anyone know a way round this? Many thanks.

Benny K C Lo
PhD Candidate
Cancer Research Laboratories
University of Nottingham
United Kingdom
Email: benny at

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