terminal transferase on cDNA

Student mucineer at iname.com
Wed Sep 29 04:19:56 EST 1999


I encountered a problem a long time ago with regard to putting poly-d(G)s at
the 5' end of cDNA by terminal transferase. However, I have been having
problem in PCRing the product with my poly(C)17 primer and another primer.
Does anyone have experience on this type of reaction? Thanks.

---
Benny K C Lo
PhD Candidate
Cancer Research Laboratories
University of Nottingham
NOTTINGHAM  NG7 2RD
United Kingdom
Email: benny at holmes.nott.ac.uk





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