Digestion of PCR-product

.Krimer Alejandro a.krimer at biosidus.com.ar
Wed Sep 29 07:34:10 EST 1999


What's about the buffer??????????
Alejandro

> -----Mensaje original-----
> De:	Eduard Post [SMTP:e.post at biol.rug.nl]
> Enviado el:	Wednesday, September 29, 1999 8:26 AM
> Para:	methods at net.bio.net
> Asunto:	Digestion of PCR-product
> 
> Hello,
> 
> I'm having problems in my restricition digest of fluorescent-labeld
> PCR-product!
> 
> There is a large undigested portion, I can't get it to be digested.
> 
> this is my digestion mix:
> 5 microliter of PCR-Product (purified)
> 10 units of AluI or HhaI
> 1.5 microliter BSA
> 
> the total volume is 7.5 microliters of mix.
> 
> Please does anyone have the same problems or does know a solution?
> 
> eduard
> 
> 



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