Digestion of PCR-product

Chunxin Wang cxwang at WAM.UMD.EDU
Wed Sep 29 08:51:39 EST 1999


seems that you didn't include the digestion buffer in your rxn. Is this
explanation too simple?:_)

Black



On Wed, 29 Sep 1999, Eduard Post wrote:

> Hello,
> 
> I'm having problems in my restricition digest of fluorescent-labeld
> PCR-product!
> 
> There is a large undigested portion, I can't get it to be digested.
> 
> this is my digestion mix:
> 5 microliter of PCR-Product (purified)
> 10 units of AluI or HhaI
> 1.5 microliter BSA
> 
> the total volume is 7.5 microliters of mix.
> 
> Please does anyone have the same problems or does know a solution?
> 
> eduard
> 
> 
> 
> 




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