Transfecting B cell lines

SHAHEEN S SUTTERWALA sutterwa at mail.med.upenn.edu
Wed Sep 29 17:39:59 EST 1999


I'm trying to stably transfect mouse A20 B cells and human .45 B cells. I 
have tried electroporation but with no success. The web site for quiagen 
says that they transfected a mouse B cell line using their transfection 
reagent - effectene. has anyone had any luck transfecting B cell lines 
with any of the commercially available transfection reagents?

Thanks

Shaheen



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