Digestion of PCR-product
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Sep 29 09:06:01 EST 1999
In article <37F1F72A.8C951F2B at biol.rug.nl>, Eduard Post
<e.post at biol.rug.nl> writes
>I'm having problems in my restricition digest of fluorescent-labeld
>There is a large undigested portion, I can't get it to be digested.
>this is my digestion mix:
>5 microliter of PCR-Product (purified)
>10 units of AluI or HhaI
>1.5 microliter BSA
>the total volume is 7.5 microliters of mix.
>Please does anyone have the same problems or does know a solution?
Where is your fluorescent label. Could it be sterically hindering the
action of the restriction enzyme at it's cleavage site?
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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