Digestion of PCR-product

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Sep 29 09:06:01 EST 1999


In article <37F1F72A.8C951F2B at biol.rug.nl>, Eduard Post
<e.post at biol.rug.nl> writes
>Hello,
>
>I'm having problems in my restricition digest of fluorescent-labeld
>PCR-product!
>
>There is a large undigested portion, I can't get it to be digested.
>
>this is my digestion mix:
>5 microliter of PCR-Product (purified)
>10 units of AluI or HhaI
>1.5 microliter BSA
>
>the total volume is 7.5 microliters of mix.
>
>Please does anyone have the same problems or does know a solution?
>
>eduard
>
>

Where is your fluorescent label. Could it be sterically hindering the
action of the restriction enzyme at it's cleavage site?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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