Protease inhibitors for yeast

[John Thompson]

Frank,

If by yeast you mean Saccharomyces cerevisiae,  it also helps to use a
pep4 or even better a pep4 prb1 mutant strain.  My favorite such
strain is BJ3501 from E. Jones lab which you can obtain from ATCC
(208280).  I'd still throw in the protease inhibitors too.
(http://phage.atcc.org/cgi-bin/searchengine/longview.cgi?view=fy,14683963&text=pep4)

Regards,
John Thompson 
Merck Research Laboratories


"Frank Stapleton" <rand106 at hotmail.com> wrote:

>Hi,
>
>I need to prepare a subcellular fraction from yeast and wonder which would
>be the best set of protease inhibitors to use.
>
>Thanks
>
>Frank
>