Protease inhibitors for yeast

John Thompson jrt at
Sat Apr 1 20:07:28 EST 2000


If by yeast you mean Saccharomyces cerevisiae,  it also helps to use a
pep4 or even better a pep4 prb1 mutant strain.  My favorite such
strain is BJ3501 from E. Jones lab which you can obtain from ATCC
(208280).  I'd still throw in the protease inhibitors too.

John Thompson 
Merck Research Laboratories

"Frank Stapleton" <rand106 at> wrote:

>I need to prepare a subcellular fraction from yeast and wonder which would
>be the best set of protease inhibitors to use.

More information about the Methods mailing list