Yellow MOPS - why?

John Thompson jrt at home.com
Sat Apr 1 20:35:23 EST 2000


Frederik Börnke <ricky_boernke at gmx.net> wrote:

>we don't care
>about anything like autoclaving or RNAes, using formamide and
>formaldehyde within the gel
>and within the loading buffer, the whole thing happens under denaturing
>conditions, so why
>bohter?
>

Agreed.  In one low budget lab that couldn't afford separate RNA
gelboxes, I even started running formaldehyde gels in tanks used with
RNAse treated minipreps without any prior decontamination... no
problem.

John Thompson
Merck Research Labs





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