How to calculate molar extinction coeff. for proteins - Extinction Coeff.xls (0/1)

John Thompson jrt at
Sat Apr 1 20:56:44 EST 2000

See Gill, S. C. and P. H. Von Hippel.  Anal Biochem. 182: 319-326,

Their equation is implemented in the attached Excel97 spreadsheet.

John Thompson
Merck Research Labs

"Student" <mucineer at> wrote:

>How should one calculate the molar extinction coeff for proteins (for
>working out concentration using OD280)? Is there any program on the web
>(free) that works that out for you when you input the sequence? THanks.

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