RNase / Gel shift

Susanne Rohrer srohrer at immv.unizh.ch
Mon Apr 3 05:45:44 EST 2000


Hi all,

reading a recent post, it seems that short RNA is less susceptible to
degradation by RNases. Is that correct?

Q2: I'll try to do a Gel shift with in vitro translated tRNA and
recombinant protein. What should I do to protect the RNA from RNases in
the protein prep? Will it be sufficiently pure after Ni-chromatography?

Susanne





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