Heating Northerns Instead of Formaldehyde

Chris LaRosa clarosa at biocomp.unl.edu
Mon Apr 3 12:39:54 EST 2000


Biotechniques just published a note where you use hot buffer instead of
formaldehyde.   It sounds less hazardous and they claimed better
resolution and sensitivity.   I wonder if anyone has experience with
this technique, and I wonder if the formaldehyde is omitted , if the
RNAses begin to muck things up?

PCPhD




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