quick colony hybridisation method?

Chris LaRosa clarosa at biocomp.unl.edu
Mon Apr 3 15:12:39 EST 2000





> 
> thanks
> Kim
I just went through the same problem.   My solution was to transform,
make a master replica on nitrocelulose, grow this, then make replicas
from the cells grown on the nitrocellose.   Then the colonies stayed on
the filters in a more homogeneous way.    Lifting colonies off agar did
result in very uneven transfer, but I tried to reduce background false
positives by hybridizing for only 4 hours.




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