quick colony hybridisation method?
clarosa at biocomp.unl.edu
Mon Apr 3 15:12:39 EST 2000
I just went through the same problem. My solution was to transform,
make a master replica on nitrocelulose, grow this, then make replicas
from the cells grown on the nitrocellose. Then the colonies stayed on
the filters in a more homogeneous way. Lifting colonies off agar did
result in very uneven transfer, but I tried to reduce background false
positives by hybridizing for only 4 hours.
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