Fluorimeter vs. Spectrophotometer for DNA quantification

Chris LaRosa clarosa at biocomp.unl.edu
Mon Apr 3 17:20:04 EST 2000



moths at my-deja.com wrote:
> 
> What are the pros and cons of using fluorimeters
> vs. spectrophotometers for DNA quantification?
> 
>PROs
 
Fluorimeter is at least 100 fold more sensitive depending on the dye you
use....

Fluorimeter is much more specific as there are dyes that bind DNA...Or
RNA

Cons....

Expense.....instrument ....and dyes.




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