gradient mini gels precast in plastic ??

[Lee Hunt]

This is less of a problem with the biorad gels but I find I can only get perhaps
30ul in the 50ul well variety
Both Flowgen and Sigma gels are even harder to get out of their casing intact

"A.F. Simpson" wrote:

> .
>
> I used Sigma 7.5% SDS-PAGE gels precast in plastic recently and found
> them highly unsatisfactory.  Three out of the five gels had incompletely
> polymerizied wells, resulting in my being able to run fewer lanes than I
> had planned.  Taking the gels apart at the end of the run was tricky and
>