gradient mini gels precast in plastic ??
c.rudroff at netcologne.de
Wed Apr 5 06:25:10 EST 2000
Von: A.F. Simpson <AFS7 at le.ac.uk>
An: methods at hgmp.mrc.ac.uk <methods at hgmp.mrc.ac.uk>
Datum: Dienstag, 4. April 2000 13:52
Betreff: Re: gradient mini gels precast in plastic ??
I used the gels from NOVEX (now Invitrogen) and have the best results ever.
Even if compared with precast glass gels.
>> hi people,
>> We noticed that some companies are providing gradient minigels precast
>> plastic (not glass, as we were used to) These gels are far less expensive
>> than the glass ones, and so we would resort to them unless for our
>> use (westerns ) some problems may have been experienced by others.
>I used Sigma 7.5% SDS-PAGE gels precast in plastic recently and found
>them highly unsatisfactory. Three out of the five gels had incompletely
>polymerizied wells, resulting in my being able to run fewer lanes than I
>had planned. Taking the gels apart at the end of the run was tricky and
>a couple of times resulted in sharp-edged bits of plastic snapping off
>Bear in mind Christina's remarks about the gels smearing if used beyond
>the expiry date. The Sigma gels I used were several weeks _within_ the
>use by date and they all smeared horribly. This despite very thorough
>rinsing of the wells before use. During the first run I thought my
>protein samples were to blame; in a second run hand cast gels run at the
>same time with the same samples did not smear at all whilst the Sigma
>gels were as bad as the first time.
>These were not gradient gels, but the problems I experienced were not
>really related to the actualy gel, rather to the format and perhaps to
>Sigma quality control. I sha'n't be buying any more.
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