X-gal stain problems

[Nick Theodorakis]

In article
<4213FFACFB2CD31198A100805FEA9B4F1667FD at lscserv1.na.lifetech.com>,
  KEvans at lifetech.com ("Evans, Krista") wrote:
> Has anyone ever seen problems with eukaryotic cells staining light
blue with
> X-gal staining? These control cells have not been transfected and
should not
> be expressing bacterial Beta-galactosidase.
>

What about lactase? Is eukarotic lactase able to hydrolyze X-gal? (Are
they intestinal epithelial cells?)

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_______________________________________________
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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