Problem PCR

Mackay, John-F {~Auckland} JOHN-F.MACKAY at roche.com
Fri Apr 7 00:07:21 EST 2000


Hmm - the wonder betaine didn't cut it eh? There was another trick from NAR
where they denatured their DNA with NaOH and then  neutralised it before
adding it to the PCR. Worth trying??? As Duncan said, enzyme blend would
also help. Another combination used (can't remember the paper) is TMAC
(60mM) and formamide (2.5%) thay may be useful.

John
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