HomeMade spin columns for 1.5 mL?

john f hess jfhess at dcn.davis.ca.us
Fri Apr 7 14:46:00 EST 2000

back in the dark ages, it was possible to put poke a small (25guage) hole
in the bottom of a 500 ul tube, add a small tuft of glass wool, then put
G50 on top.  Put the small tube in a 1.5 ml tube (without a hole!).  A
couple trial spins to pack the G50 and then you have a small column to
sepearate nukes from incorporated.  I haven't done it in 10 years, and
can't remember much more detail.


In article <38EE17C5.BE04C3C2 at biocomp.unl.edu>, Chris LaRosa
<clarosa at biocomp.unl.edu> wrote:

> A web protocol on use of diatomaceous earth for plasmid miniprep
> mentioned, without details of making spin column by poking hole with
> needle in 1.5 ml eppendorf tube.... Can anyone fill in more detail what
> this might entail?
> PC LaRosa, Long Suffering Ph.D.

John F. Hess                                      jfhess at ucdavis.edu
Dept of Cell Biology and Human Anatomy                
School of Medicine
Davis, CA 95616-8643

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