Problem PCR

ChenHA hzhen at freeuk.com
Sun Apr 9 12:34:13 EST 2000


"Mackay, John-F {~Auckland}" wrote:
> 
> Hmm - the wonder betaine didn't cut it eh? There was another trick from NAR
> where they denatured their DNA with NaOH and then  neutralised it before
> adding it to the PCR. Worth trying??? As Duncan said, enzyme blend would
> also help. Another combination used (can't remember the paper) is TMAC
> (60mM) and formamide (2.5%) thay may be useful.
> 

I don't know if the reference is in there, but the following
website gives a good list of additives that you can use:
http://taxonomy.zoology.gla.ac.uk/~rcruicks/additives.html

> John
> ---




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