Vacuum Blotter - RNA transfer

David L. Haviland, PhD dhavilan at imm2.imm.uth.tmc.edu
Sun Apr 9 20:31:55 EST 2000


On 9 Apr 2000, VASANTHARAJAN JANAKIRAMAN wrote:

> After fiddling with the set up-porous screen with the gaskets, last time
> after 3 hrs. (in 10xssc), all my wheat RNA got transferred into the hybond
> N+ membrane.
> vasanth

Anyone tried NaPO4 transfer with a vacuum blotter?  We do it all the time
with the Altec transfer stones and it works very well.  We have a vacuum
blotter but will try a Northern transfer soon or next time.

Just curious,
David
==========================
David L. Haviland, PhD
Assistant Professor, Immunology
University of Texas, HSC - Houston
Institute of Molecular Medicine
2121 W. Holcombe Blvd.
Houston, TX  77030
http://www.uth.tmc.edu/~dhavilan
713.500.2413-Voice
713.500.2424-FAX
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