Acrylamide gel expansion during staining

Raymond Dalgleish ray at le.ac.uk
Mon Apr 10 07:04:53 EST 2000


We are experiencing problems while staining
polacrylamide gels with ethdium bromide. The gels
are CSGE gels for mutation detection as described by Jarmo Körrkö at

http://www.auhs.edu/medschool/centers/matrix/CSGE.htm

The recommendation is that the gel be stained with ethidium bromide in
water. In our experience,
the gel expands dramatically and becomes wrinkled and folded.

What can we do to prevent the expansion? We will
try staining in gel buffer + ethidium next time. Anybody got any other
ideas?

Thanks,

Raymond Dalgleish






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