Silencing the tac promotor?

Edgar Valencia edgar at cifn.unam.mx
Tue Apr 11 10:02:12 EST 2000


Hi Yoram

You can try the glucose trick, you can also put and additional represor
(lacIq), clone some transcriptional terminators between the promoter and
your gene.
I tried all of the above with a plasmid having either the pTac or the
ptaclacUV5 promoter. I was working with Rhizobium and unfortunately none
of them worked in my system, but seems all of them work to a variable
extent in E. coli, so, take your pick.
Good luck
Edgar

> Dear Netters
> Is there a way to stop expression from tac promotor (in E.coli) in a
> reversible way? Will addition of glucose in minimal media do the trick?
> Yoram
> .........................................................................
> "Support bacteria - they're the only culture some people have"


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