Acrylamide gel expansion during staining

Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Apr 12 10:35:30 EST 2000


I am not sure about this in this particular case....

At 12:29 PM 4/12/00 GMT, Jonathan Warawa wrote:
>I also have found that water will cause PAGE gels to expand, but a
>solution of 45%water, 45%methanol, 10% acetic acid  will make a gel shrink
>(staining solution used for coomassie).  Perhaps a mix of the two
>solutions will maintain gel size.
>Good luck
>
>Raymond Dalgleish wrote:
>
>> We are experiencing problems while staining
>> polacrylamide gels with ethdium bromide. The gels
>> are CSGE gels for mutation detection as described by Jarmo Körrkö at
>>
>> http://www.auhs.edu/medschool/centers/matrix/CSGE.htm
>>
>> The recommendation is that the gel be stained with ethidium bromide in
>> water. In our experience,
>> the gel expands dramatically and becomes wrinkled and folded.
>>
>> What can we do to prevent the expansion? We will
>> try staining in gel buffer + ethidium next time. Anybody got any other
>> ideas?
>>
>> Thanks,
>>
>> Raymond Dalgleish
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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