expression of transmembrane transporter in E. coli

ChenHA hzhen at freeuk.com
Thu Apr 13 19:32:52 EST 2000


Wolfgang Schechinger wrote:
> 
> Hi all,
> 
> I wonder if I (maybe I should write E. coli) will face problems when
> expressing a translocable eucaryotic passive transporter protein in
> bacteria. Will it insert into E. coli's cell walls and maybe kill the
> poor bug (especially when the transporter's target substance is / is
> not present in the bacterial growth medium / agar plate?)
>

Working on membrane protein? You have my sympathy. I would
expect problems in expressing membrane proteins in coli (or
indeed any type of cells), although I don't think absence of
target substance would affect the cells (I suspect it might
actually be better - it's generally better for a
heterologous protein not to be actively affecting the
metabolism of the host cell).  If you haven't done so
already, do read the following:

von Heijne, J Mol Biol 1999 Oct 22;293(2):367-7  

It's great fun, though I suspect not if you are one of those
working on membrane proteins.  It has lots of useful
references in there though.
 
> All input is welcome!
> 
> Wolfgang
> -----
> This message is encrypted. Use your brain to decode it.
> -----
> Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
> University of Tuebingen, Germany
> email: wolfgang.schechinger at med.uni-tuebingen.de
> wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
> -----
> usual disclaimers apply
> -----
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